RESUMO
This study was designed to evaluate the absolute CD4+ counts and percentages in HIV subtype C infected patients at a tertiary care hospital in northern India. The CD4+ counts of 377 HIV seropositive subjects were estimated by a FACS Calibur (BD) flow cytometer. Dual color immunophenotyping was performed on each sample, which was acquired and analysed using CellQUEST software. Discordance between CD4+ counts and percentages were found more in the early stage ie Group A (37.2%) when compared with Group B (31.6%) and Group C (28.8%), with the counts remaining in the normal range but percentages being severely depressed.
Assuntos
Contagem de Linfócito CD4 , Relação CD4-CD8 , Infecções por HIV/epidemiologia , Infecções por HIV/imunologia , Adolescente , Adulto , Idoso , Fármacos Anti-HIV/uso terapêutico , Biomarcadores/análise , Linfócitos T CD4-Positivos/imunologia , Estudos de Coortes , Feminino , Citometria de Fluxo , Infecções por HIV/diagnóstico , Infecções por HIV/tratamento farmacológico , Soropositividade para HIV/diagnóstico , Soropositividade para HIV/epidemiologia , Soropositividade para HIV/imunologia , Humanos , Índia/epidemiologia , Masculino , Pessoa de Meia-Idade , Monitorização Fisiológica , Prognóstico , Sensibilidade e Especificidade , Índice de Gravidade de Doença , Distribuição por Sexo , Resultado do Tratamento , Carga ViralRESUMO
BACKGROUND: As the number of AIDS cases increases in India, information available among clinicians about the prevalence of opportunistic infections (OIs) is scarce. The aim of the present study was to document the characteristic OIs of HIV-infected North Indian patients along with their CD4+ counts. PATIENTS AND METHODS: The study group consisted of subjects with confirmed serodiagnosis of HIV, attending the medical clinics at a tertiary health care center in North India. The CD4+ counts were estimated by FACS Calibur (BD) flow cytometer. Simultaneously, routine microbiology smears, cultures and serology were performed to confirm OI. RESULTS: In this retrospective study of 421 subjects, the predominant OI was tuberculosis (47%, 189 cells/ micro l), followed by parasitic diarrhea (43.5%, 227 cells/ micro l) and oral candidiasis (25.2%, 189 cells/ micro l). CONCLUSION: Tuberculosis was the most frequent OI in the HIV-infected patients studied, and the major mode of transmission of HIV was by sexual route. The median CD4+ counts observed were lower when compared to other studies.
